Pharmacology and Toxicology of Plant Extracts
Many factors influence the composition and activity of plant extracts, resulting in differences in their toxicological and pharmacological effects. Lifeasible can perform pharmacological and toxicological tests on different extracts to assess the maximum oral tolerance of various plant extracts in female and male ICR mice. And whether its intake in the dose range has any significant effect on the growth and development, food utilization, blood system, liver, kidney function, reproductive system, and protein, fat, and sugar metabolism of experimental rats. To evaluate whether the extract shows acute toxicity, genotoxicity, and sub-chronic toxicity under certain experimental conditions.
Our Services for Pharmacology and Toxicology
Acute Oral Toxicity Test
The acute oral toxicity test is the first step in evaluating the toxic properties of plant extracts. The acute oral toxicity of the samples can be evaluated and graded by the acute oral toxicity test and LD50 determination in a stepwise manner using a small number of animals. This can be used as a basis for toxicity classification and labeling of plant extracts as well as for determining doses for subchronic toxicity tests and other toxicological tests.
Genotoxicity Test (Rat/Mouse Bone Marrow Cell Micronucleus Test)
We provide rat/mouse bone marrow cell micronucleus test results with high confidence, which can detect chromosomal abnormal effects for a long time and can detect spindle toxins. The background of the specimen is clear, and chemicals other than the compound being tested are not required.
a) Rats/mice dosed with compound, 3 doses, 6 animals/group. Animals are sacrificed 24 or 48 hours later.
b) Micronuclei may be formed by the loss of whole chromosomes during division or by chromosome breakage. The erythrocyte's nucleus is extruded leaving any micronuclei behind.
c) Femurs were removed and bone marrow was aspirated.
d) Bone marrow cells spread onto slides. Slides fixed and stained (acridine orange).
e) 2,000 cells were analyzed per animal, number of micronucleated immature erythrocytes was scored.
Subchronic Toxicity Test (90-Day Feeding Test)
Subchronic toxicity testing allows us to explore the threshold dose or concentration for subchronic toxicity and the dose level at which no toxic effects are observed during the subchronic test, and to find exposure doses and observations for chronic testing.
Other Tests Available
| Mouse sperm aberration test | Ames test |
| 30-day feeding test in rats | Teratogenic test |
| Immune enhancement test | Adjuvant lipid-lowering test |
| Auxiliary hypoglycemic test | Auxiliary protection against chemical liver injury test |
| Laxative function test | Promoting lead excretion function test |
| Antioxidant function test (delayed aging function test) | Throat clearing function test |
| Skin allergy test | Vaginal mucous membrane irritation test |
| One-time skin breakage test | One-time skin irritation test |
| Multiple skin irritation test | Conjunctival Irritation Test |
Applications
- Obtaining plant extracts with higher pharmacological activity.
- Improve quality control of plant extracts.
- Differentiate plant extracts from different sources.
Lifeasible specializes in providing you with the applicability of assays for different target plant extracts for a variety of toxicity endpoints including reproductive developmental toxicity, endocrine disruption toxicity, high potential carcinogens, groups of concern, organophosphates, and carbamates. This means that we can provide you with a customized service based on your project requirements, so please feel free to contact our staff for information.
※ For research or industrial raw materials, not for personal medical use!