Antiviral Mechanism Study

Plant extracts have a wide range of applications, including food, medicine, feed, cosmetics, and so on. Natural plant extracts have strong biological activities, such as immunomodulation, anti-infection, enhanced antioxidant capacity, anti-inflammatory, and other effects, and the exploration of their medicinal value has been of great interest to researchers.

Lifeasible can assist you in obtaining antiviral components from plant extracts by providing you with antiviral activity screening and in vivo/ex vivo antiviral pharmacodynamic assays. To guide for you to prioritize the screening of antiviral agents from plant extracts with antiviral activity, and to improve the success rate and utilization of plant resources for antiviral screening.

Our Services for Antiviral Mechanism Study

Screening for Antiviral Activity (in the field of pesticide research)

Using Tobacco mosaic virus (TMV) as a model, we can screen a variety of plant extracts for antiviral activity, helping you to identify good antiviral plant resources.

• We determined the inhibitory effect of plant extracts against TMV infestation in vitro using the half-leaf blight method.
• We used the leaf disc suspension screening method to determine the anti-TMV proliferative effect of plant extracts in vivo.

General Procedure for The Preparation of Antiviral Drugs

• The extract was concentrated and precipitated with ethanol to obtain the supernatant.
• The supernatant was concentrated and adsorbed by macroporous resin, and the column was washed with ethanol to collect the eluate.
• The eluate was concentrated under reduced pressure and dried to obtain the antiviral active part.

In vitro Antiviral Assay

• In vitro antiviral activity screening (CC50, EC50).
• In vitro screening and evaluation index (SI).

In vivo Pharmacodynamic Tests

We provide animal infection models to evaluate the in vivo antiviral activity of plant extracts. The requirements for viral strains and subjects are the same as those for in vitro antiviral tests.

We provide in vivo pharmacodynamic evaluation indexes including, but not limited to, general observations (mortality, weight change, clinical signs, etc.), viral load, gross examination, histopathology, hematology, and blood biochemistry indexes (if necessary), and so on. Of these, viral load and histopathologic examination are the key indicators. If necessary, the blood concentration of the drug in the animal and/or the concentration of the drug localized to the infection should be determined to indicate the relationship between effectiveness and drug exposure.

Lifeasible can predict whether a plant extract will achieve effective antiviral concentrations in vivo in combination with the results of other studies (e.g., protein binding, tissue distribution, pharmacokinetics) when only the results of in vitro antiviral tests are available. If necessary, we also support you in conducting phased resistance studies, screening for resistant virus strains, genotyping/phenotyping of resistant strains, and cross-resistance studies.

Please feel free to contact us for more information.

※ For research or industrial raw materials, not for personal medical use!