Acute Toxicity Assessment
Plant extracts have a wide range of applications in food, medicine, and other fields. In natural drug development, acute toxicity tests are to evaluate the toxic response exhibited by animals after single or multiple cumulative administrations within 24 hours, which is the first step of drug safety evaluation. Lifeasible conducts acute and sub-acute toxicity studies on plant extracts to provide toxicological technical support for the development, utilization, and safety evaluation of plant extracts.
Our Services
- Acute toxic effects of plant extracts
We can determine the lethal dose of 50% (LD50) and the maximal tolerance dose (MTD). The acute toxicity of plant extracts in mice can be observed by administering the maximum concentration and maximum mouse gavage volume. Response doses such as maximum administered dose, maximum non-toxic response dose, maximum tolerated dose, and lethal dose can generally be determined. - Determination of MTD in mice
- Mouse organ index calculation
- Acute toxicity MTD body mass change in mice
- Sub-acute toxicity of plant extracts
To observe whether plant extracts have toxic effects when consumed for a long period, we can further do sub-acute toxicity experiments. We analyze the effects of extracts on liver and kidney function, organ index, intake curve, and body weight changes in mice to support you in dose setting and adverse reaction monitoring of plant extract tests, which can be used to preliminarily evaluate the safety of plant extracts.
- Use of zebrafish embryos to test the acute toxicity of plant extracts
In addition to using mice as indicator organisms, we can test the acute toxicity of plant extracts using the embryos of the model animal zebrafish. We observed and recorded the effects of different concentrations of dilutions on zebrafish embryo development to determine the toxicity of plant extracts on zebrafish embryo development. We use this method to establish an early, sensitive, and high-throughput screening acute toxicity assay platform, which is easy to operate, practical, fast, and accurate and provides a new option for determining the acute toxicity of plant extracts. - Zebrafish culture
- Embryo selection
- Preparation of embryo culture medium
- Statistics on transparency, survival rate, average malformation rate
Why Choose Us?
Lifeasible provides randomized, controlled, and replicated test designs with blank and/or negative control groups to ensure accurate and reliable results. Acute toxicity tests are conducted using different routes of administration to maximize the observation of acute toxicity in animals, as the rate of absorption, speed of uptake, and exposure may vary depending on the route of administration. Reliable results and a dedicated team of professionals you can trust, please feel free to contact us to start your project as soon as possible.
※ For research or industrial raw materials, not for personal medical use!