Cytotoxicity Assessment
Specific extracts may have strong chemosensitizing effects on a wide range of plants, significantly inhibiting plant growth and development, and have the potential to be developed into plant-derived herbicides for green weed control. In addition, plant extracts with cell proliferation inhibitory properties have the potential to be used in the development of anti-inflammatory, antibacterial, antitumor, immunomodulatory, and antiparasitic drugs. Lifeasible offers a wide range of assays to accurately detect cytotoxicity and antiproliferative effects of your plant extracts.
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We examined the cytotoxicity of the extracts by sulphonyl rhodamine B (SRB) assay, lactate dehydrogenase (LDH) assay, and MTT assay.
- SRB assay
SRB is a water-soluble protein-dye that binds to basic amino acids of intracellular proteins to form complexes, and the amount of total protein bound to the cells reflects the number of cells. We quantified the cell number by measuring the absorbance at 515 nm.
- LDH assay
LDH is a stable cytosolic enzyme in all cells and is rapidly released into the cell culture fluid when the cell membrane is damaged. We can determine the extent of cell damage by measuring LDH activity in cell culture supernatants. This method has the advantages of sensitivity, convenience, and accuracy of analysis and is suitable for cytotoxicity analysis of different species of plant extracts.
- MTT assay
The MTT assay is based on the principle that the enzyme succinate dehydrogenase (SDH) in the mitochondria of living cells reduces exogenous MTT to the water-insoluble blue-violet crystalline formazan and deposits it in the cells, whereas dead cells do not have this function. We will determine the number of viable cells based on the measured absorbance value (OD value). The higher the OD value, the more cellular activity indicates less cytotoxicity of the tested plant extract.
We offer both cancerous and non-cancerous cell lines for use in the determination of plant extract cytotoxicity. These cell lines include HT-29, Hep3B, MCF-7, SH-SY5Y, SAOS-2, LNCap, and Vero.
- Other optional indicators for cytotoxicity analysis of plant extracts:
- We examined the effect of extracts on mitochondrial membrane potential in the indicated cells using JC-1 as a marker.
- We evaluated the redox status of the indicator cells by measuring glutathione (GSH) accumulation.
- We determined extract-induced apoptosis or cell death by staining with membrane-linked protein V-propidium iodide (PI) and by flow cytometry.
- We used a bioluminescence assay to determine the effect of the extract on the amount of ATPase in the indicator cells.
Why Choose Us?
Lifeasible can help you analyze the cytotoxicity of plant extracts. We can not only isolate and purify the active ingredients in plant extracts, but also detoxify them according to your needs, and evaluate the biological activity and safety of the resulting products. Please feel free to contact our staff to submit your requirements, so that we can realize the comprehensive utilization of plant extract resources as soon as possible.
※ For research or industrial raw materials, not for personal medical use!