Antioxidant Activity Assay
In plant extract testing programs, antioxidant activity is one of the key indicators for evaluating plant extracts for product development and quality control. To help you develop high-value-added plant extracts that can be widely used in the food, nutraceutical, and pharmaceutical industries, Lifeasible can help you understand the anti-aging, anti-fatigue, anti-inflammatory, and other biological activities of your target extracts through testing.
Our Services for Antioxidant Activity Assay
Currently, the methods we use to evaluate antioxidant activity in plant extracts are categorized into in vivo and in vitro methods, depending on the subject. The in vivo method has the advantage of being closer to the human body results, but generally can only reflect the level of activity of a certain index. The in vitro method makes up for the shortcomings of the in vivo method.
- The in vivo method uses malondialdehyde, superoxide dismutase, glutathione, etc. as indicators, and their contents are measured to evaluate the level of antioxidant activity in vivo.
- The in vitro antioxidant evaluation system is based on free radical scavenging and redox methods.
We have a wide range of representative assays for you to choose from, among which we have established an antioxidant analytical method based on thin-layer chromatography (TLC) coupled with DPPH colorimetric reaction. This method is highly selective and versatile and can be used as an analytical platform for the efficient integration of multiple off-line detection methods. The analytical fluence, simplicity, precision, and applicability have reached an ideal balance.
a) Extract the antioxidant active ingredients in the sample using methanol as solvent (the solvent used in this step is not fixed and is related to the sample you provide).
b) Extraction is assisted by ultrasound.
c) A silica gel thin layer plate was used as a stationary phase.
d) Ethyl acetate, acetic acid, formic acid, and water were used as mobile phases.
e) Impregnate DPPH solution for color development.
f) Scanning is performed in fluorescence mode with a data resolution of 100 μm/step.
We used silica gel TLC to unfold the methanol solution of the plant extracts to achieve chromatographic separation of various antioxidant substances therein. The separation results resting on a thin layer of silica gel were then coupled to the DPPH color reaction by impregnation. The results of the color reaction were quantified by an optical density scanner. The assay results were weighted and quantitatively calculated to assess the combined antioxidant capacity of the samples using suitable standards as internal standards. By analyzing the molecular structure characteristics of representative antioxidant active compounds, we can optimize the key technical parameters such as chromatographic separation and DPPH color development.
Why Choose Us?
Lifeasible has a complete instrumentation platform for a wide range of applications with semi- or fully automated processes for dispensing, unfolding, dip derivatization, and scanning for quantification. In the analysis of the antioxidant activity of plant extracts, all steps are independent and equipped for simultaneous operation and analysis, which not only provides high efficiency but also increases the flexibility of your choice of instrumentation. Our instrumented platforms provide a high degree of sensitivity for chromatographic separations and resolution of chromatograms, while optical density scanning after visual imaging improves the accuracy and reproducibility of results.
Please feel free to contact us to submit your requirements.
※ For research or industrial raw materials, not for personal medical use!